About transfer volumes in instrument configuration

In the instrument configuration, you provide a transfer volume:

Configuration of detector transfer times in a 2D-LC system
Figure Configuration of detector transfer times in a 2D-LC system

By default, this detectors table lists only CAN-based detectors. If you have connected an MSD or ELSD detector, you must add those detectors and their transfer volumes manually into the detectors table.

NOTE

Ensure that you use the exact module identifier (detector name and serial number, separated by colon). Otherwise, the detector module cannot be identified, and the Data Delay (min) will be displayed as N/A in the processing method. In this case you have to align signals manually in the processing method (see Align signals manually).

The same detectors are, among others, also listed in the processing method under General > Signals > Alignment .

Transfer volume and transfer time

In the processing method, the Data Delay (min) corresponds to the detector transfer volume from the instrument configuration, divided by the respective pump flow. Therefore, the delay time can be seen as a transfer time.

Detectors in the processing method and in instrument configuration
Figure Detectors in the processing method and in instrument configuration

In the processing method, all detectors are listed that are used in the data linked to the this method. If the method is not linked, and you have loaded different data from different instrument configurations with different transfer volumes, then the table in processing method displays various under Data Delay (min).

Difference to regular (1D) Data Analysis

In regular (1D) Data Analysis, only the delay (retention time difference) between two detectors has to be specified in the signal alignment. For the first ¹D detector, this value is always zero. In 2D-LC, the transfer time in the first dimension is measured from the detector to the 2D-LC valve.

See Also

Align signals over two dimensions

Learn more about signal alignment in the core Data Analysis.

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