Valve

Mainpass

Turns the valve to the main flow path position

Main+Start

Turns the valve to the main flow path position and generates a start pulse.

NOTE: The VALVE function with Main+Start parameter is not allowed in conjunction with overlapped injection.

Bypass

Turns the valve to the bypass position.

Main In

Turns the valve to the main flow path position while the needle is at the Source for a Time between 1 and 99 seconds. The source (from can be a Sample, a Location, Seat, the wash port, Sample+ or Vial+.

Sample

The position specified in the sample information or sequence/worklist.

Location

A specified location. The location can be a well or a vial.

See Specifying a Location.

Seat

The seat capillary.

Washport

The wash port.

Sample+

Sample+ is used for wellplate sample locations only; it defines an offset from the current location. You specify the offsets for Tray, Plate, Row and Column individually. For example, if the current location is P1-A-01, values for Plate, Row and Column of 1 define location P(1+1)-(A+1)-(01+1), which is P2-B-02. The limits are:

Tray: -1 to +1 (only valid if two trays are inserted)

Plate: -2 to +2 (only valid for wellplates)

Row: -15 to +15

Column: -23 to +23

NOTE: Sample+ may not be used with Overlapped Injection.

Vial+

This function is valid for vial trays only.

Vial+ is used for sample vial locations only; it defines an offset from the current sample vial number. For example, if the current sample vial is 1, a value for Vial+ of 3 specifies a vial that is three positions away from the sample vial, in this case vial 4. The limits are independent of the sample tray configuration:

-99 to +99

NOTE: Vial+ may not be used with Overlapped Injection.

to (only for Sample, Location, Sample+ and Vial+) is the time to leave the valve in the main flow path (in seconds, range is 0 – 9999)

use Offset (only for Sample, Location, Sample+ and Vial+) is the vertical offset of the needle relative to the default position. Range is Default as defined in Draw Position in the method, or -10 – 50.

The offset of the autosampler needle is calculated from the bottom of the well, with a slight amount of clearance to cover tolerances. Use a negative offset to touch the well bottom with the needle. Use a positive offset to raise the needle higher in the well.