Needle

Up

Moves the needle to its upmost position.

Down

Moves the needle to its lowest position.

Into

Moves the needle into a specified Source with a specified Offset. The Source can be Sample, Location, Seat, Upmost, Sample+ or Vial+. The Offset of the autosampler needle is calculated from the BOTTOM of the well, with a slight amount of clearance to cover tolerances. Use a negative offset to touch the well bottom with the needle. Use a positive offset to raise the needle higher in the well to clear precipitates on the bottom of the well, or to select one phase of a biphasic sample. The unit is in millimeters, with 1/10 mm resolution. The range of offsets available is from -10 to 50 mm.

Sample

The position specified in the sample information or sequence/worklist.

Location

From a specified location. The location can be a well or a vial.

See Specifying a Location.

Seat

The seat capillary.

Upmost

The upper position of the needle. This command is equivalent to using the Up parameter.

Sample+

Sample+ is used for wellplate sample locations only; it defines an offset from the current location. You specify the offsets for Tray, Plate, Row and Column individually. For example, if the current location is P1-A-01, values for Plate, Row and Column of 1 define location P(1+1)-(A+1)-(01+1), which is P2-B-02. The limits are:

Tray: -1 to +1 (only valid if two trays are inserted)

Plate: -2 to +2 (only valid for wellplates)

Row: -15 to +15

Column: -23 to +23

NOTE: Sample+ may not be used with Overlapped Injection.

Vial+

This function is valid for vial trays only.

Vial+ is used for sample vial locations only; it defines an offset from the current sample vial number. For example, if the current sample vial is 1, a value for Vial+ of 3 specifies a vial that is three positions away from the sample vial, in this case vial 4. The limits are independent of the sample tray configuration:

-99 to +99

NOTE: Vial+ may not be used with Overlapped Injection.