This section provides three configurable Plug Modes. Click the down arrow and select the appropriate mode from the drop-down list:
The Sample plug is followed by an optional three-part Loop Solvent plug and Positioning Plug. The Loop Solvent plugs and Positioning Plug use the current solvent composition from the method. All volumes except the Sample injection volume and the solvent plug are fixed.
Specify the total volume of the solvent plug in the adjacent field; the volume you specify is divided by three for each of the parts.
Clear the check box to remove the Loop Solvent plug and Positioning Plug.
The Sample plug is followed by an optional three-part Post-sample Plug and Positioning Plug. The Post-sample Plug plug can be either pure organic or a mixture of organic and water. It provides a higher transport capacity of sample through the tubing to the injector valve for cases where the polarity of the analytes and of the loop content, which is based on the start gradient, do not match. In addition, highly concentrated samples require the use of post-sample plugs because of their elevated transport capacity. Segmentation provides increased transport efficiency and ensures quantitative sample transfer from the needle tip to the injector valve. It further minimizes risks of precipitation for samples of high concentration.
The Post-sample Plug and Positioning Plug use a different solvent from the Loop Solvent, which is the current solvent composition from the method. All volumes except the Sample injection volume and the Post-sample Plug are fixed.
Specify the total volume of the Post-sample Plug in the adjacent field; the volume you specify is divided equally between the parts. Click the down-arrow and specify the location of the solvent for the Post-sample Plug from the drop-down list; you can select from:
Location
Current Container
A user-specified location that refers to the currently used sample container, allowing you to omit the multisampler drawer to be used from the specification. In this case, it is sufficient to specify the row and column of the location to be used.
Sample+
A user-specified increment to the previously specified vial location (as specified in the sample information or sequence/worklist) by N positions; sample is drawn from the new position. Sample+ is intended for containers that support linear addressing only.
Location+
A user-specified increment to the previously specified wellplate location (as specified in the sample information or sequence/worklist) by N rows and M columns; sample is drawn from the new position. Location+ is intended for containers that support row and column addressing only.
Container
A location relative to the previously specified location (as specified in the sample information or sequence/worklist) on a different container. Location coordinates and drawer specification remain unchanged. You can select from Front, Middle or Back.
Drawer
A location relative to the previously specified location (as specified in the sample information or sequence/worklist) on a different drawer. Location coordinates and container specification remain unchanged. You can select from D1, D2 or D3.
Add a comment in the Comment column, if necessary. The Comment is limited to 20 characters; however, the use of some special characters may reduce this limit.
Clear the check box to remove the Post-sample Plug and Positioning Plug.
The Sample plug is preceded by an optional Pre-sample Sandwich Plug, and followed by an optional Post-sample Sandwich Plug. The pre-sample/post-sample sandwich plug consists of pure organic solvent and is used to protect the sample from precipitation.
These are followed by an optional three-part Post-sample Plug and Positioning Plug. In Preparative operating mode, all of these plugs should use the same solvent, although each can be set independently if required. In Analytical operating mode, theSandwich Plugs use the same kind of solvent, but different from the loop solvent. The Post-sample Plug and Positioning Plug use a different solvent from both the two Sandwich Plugs and the Loop Solvent (the current solvent composition from the method). All volumes except the Sample injection volume, Sandwich Plugs and Post-sample Plug are fixed.
Specify the volumes of the Pre-sample Sandwich Plug and Post-sample Sandwich Plug in the adjacent fields. Click the down-arrow and specify the location of the solvent for the Sandwich Plugs from the drop-down list; you can select from:
Location
Current Container
A user-specified location that refers to the currently used sample container, allowing you to omit the multisampler drawer to be used from the specification. In this case, it is sufficient to specify the row and column of the location to be used.
Sample+
A user-specified increment to the previously specified vial location (as specified in the sample information or sequence/worklist) by N positions; sample is drawn from the new position. Sample+ is intended for containers that support linear addressing only.
Location+
A user-specified increment to the previously specified wellplate location (as specified in the sample information or sequence/worklist) by N rows and M columns; sample is drawn from the new position. Location+ is intended for containers that support row and column addressing only.
Container
A location relative to the previously specified location (as specified in the sample information or sequence/worklist) on a different container. Location coordinates and drawer specification remain unchanged. You can select from Front, Middle or Back.
Drawer
A location relative to the previously specified location (as specified in the sample information or sequence/worklist) on a different drawer. Location coordinates and container specification remain unchanged. You can select from D1, D2 or D3.
Add a comment in the Comment column, if necessary. The Comment is limited to 20 characters; however, the use of some special characters may reduce this limit.
Specify the total volume of the Post-sample Plug in the adjacent field; the volume you specify is divided equally between the parts. Click the down-arrow and specify the location of the solvent for the Post-sample Plug from the drop-down list; you can select from:
Location
Current Container
A user-specified location that refers to the currently used sample container, allowing you to omit the multisampler drawer to be used from the specification. In this case, it is sufficient to specify the row and column of the location to be used.
Sample+
A user-specified increment to the previously specified vial location (as specified in the sample information or sequence/worklist) by N positions; sample is drawn from the new position. Sample+ is intended for containers that support linear addressing only.
Location+
A user-specified increment to the previously specified wellplate location (as specified in the sample information or sequence/worklist) by N rows and M columns; sample is drawn from the new position. Location+ is intended for containers that support row and column addressing only.
Container
A location relative to the previously specified location (as specified in the sample information or sequence/worklist) on a different container. Location coordinates and drawer specification remain unchanged. You can select from Front, Middle or Back.
Drawer
A location relative to the previously specified location (as specified in the sample information or sequence/worklist) on a different drawer. Location coordinates and container specification remain unchanged. You can select from D1, D2 or D3.
Add a comment in the Comment column, if necessary. The Comment is limited to 20 characters; however, the use of some special characters may reduce this limit.
Clear the check boxes to remove the Pre-sample Sandwich Plug and/or Post-sample Sandwich Plug and/or Post-sample Plug and Positioning Plug.
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