Set up a calibration (Data Analysis)
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Set up a calibration

The following procedure describes how you set the calibration parameters for a compound that is already part of the processing method.

Prerequisites

  • To be able to carry out the procedure as described, you need the privileges Processing Method > Edit calibration parameters and Data > Edit sample information. Privileges are configured in the Control Panel.

  • If the project enforces method approval, the method status must be Generic. For details, see Method approval.

In the Processing Method window:

  1. Select the Compounds > Calibration node.

  2. Select the compound you want to calibrate.

  3. If you want to change the number of levels, select the General tab and set a different number in the Number of levels text box.

  4. Provide the Amount unit for each calibration level.

    5. The amount unit is equivalent to the x-axis naming of the calibration curve.

  5. Provide the Concentration unit for each calibration level.

    6. The concentration will be calculated after calibration. It will be shown in the Injection Results window.

    To receive the correct concentration, multipliers must be set in the Injection List window.

  6. Choose if the Response used to calibrate and quantify the compound is Area, Height, Area% or Height%.

  7. Choose a Mode (for example, Curve) and a Curve model (for example, Linear).

  8. In the Origin column, choose from the following options:

    • Ignore: The origin is not used. At least two calibration points are needed.

    • Include: A virtual point (0;0) is taken into account to calculate the equation of the calibration curve. At least one calibration point is needed.

    • Force: The calibration curve is forced to the origin. The curve equation is y = ax. At least one calibration point is needed.

    • Connect: The curve is connected to the origin by a linear segment from the lowest calibration level point. At least two calibration points are needed.

    9. If your calibration curve has only one level, we recommend using the Force option. With Include, the intercept may have a very small value.

  9. If required, set a compound specific Multiplier.

In the Injection List window:

  1. Set the Sample type to Cal. Std. for each calibration standard.

  2. In Run type, you can either leave the empty space, which means the calibration point will stay in the calibration curve after reprocessing. Or you can choose between Clear all calibration or Clear calibration at level to remove certain calibration points.

  3. Under Level, define which calibration standard equals which level that you have set in the calibration parameters of your method.

  4. In Multiplier 1-5 and Dil. factor 1-5, set the correct value to calculate the concentration of your sample.

See Also