When the spotting option for the G1364D Low Flow Fraction Collector is used, the following information applies in addition to the fraction collector needle movements.
This is the recommended mode for spotting operation,
When this mode is used, the distance between plate and needle increases depending on the spot volume/height. The distance of the needle tip above the plate surface depends on flow rate, spot size and the offset of the z-axis at the beginning of the spot.
The Distance parameter determines the offset between MALDI-Plate and the needle tip at the beginning of each spot. This parameter allows fine adjustment of the spotting process.
A positive value means an offset increase.
A negative value means an offset decrease.
Like the Distance parameter for the contact control, the Contact Control Adjustment setting in the Micro Settings section of the Auxiliary set up of the method can be used to optimize the spotting process.
Repositioning of the needle using the Distance parameter or Contact Control Adjustment parameter needs to be done very carefully. Decreasing the needle offset may force the needle onto the MALDI target. |
The needle is placed over the plate as adjusted by the accuracy calibration procedure. The needle moves into the fraction location by the specified depth. The default depth value is set to 0.0 mm.
The needle movement into location cannot be operated in conjunction with continuous flow. |
This movement is not recommended for spotting applications.