Sampling Table (2D-LC)

The Sampling Table shows the 2D-LC sampling timetable in three columns.

The title bar shows the number of events in the Sampling Table and the number of available events remaining. Each additional gradient point (²D Gradient, Shifted Gradient or Flush Gradient) reduces the total number of events available in the Sampling Table.

Time

The start time of the cut.

Function

The mode of sampling, Time-based Heart cut, Time-based Comprehensive, Start Peak-based or End Peak-based. Click the down-arrow to select an alternative mode.

Start Peak-based peak detection continues until an End Peak-based or a new Time-based section is encounterd.

Parameter

(Time-based Heart-cut and Peak-based)

The parameters of the cut depend on the Function selected.. Click in the cell to change the parameters:

• MHC/HiRes

  Click the down-arrow to switch between MHC and HiRes.

• Cut size

  The length of the cut in seconds. The Cut size cannot be changed in MHC mode.

• Cut(s)

  The number of cuts. The number of cuts cannot be changed in MHC mode.

• Loop Filling

  (Time-based Heart Cut)

  The loop filling factor. This value is read-only and cannot be changed.

• Multi-Inject

  This check box is enabled when the number of cuts is more than one,

  Mark the check box to delay the analysis of multiple cuts until all cuts have been parked.

• Analyze Mode

  Click the down-arrow and choose a mode from the drop-down list:

  Default: the cut is analyzed as soon as possible.

  Delayed: analysis is delayed until there is an available time slot.

  Ignored: the cut is not analyzed.

• Prioritize cut(s)

  (Time-based Heart Cut)

  Mark this check box to specify that analysis of the cut(s) should be given priority.

• Reference Index

  Click the down-arrow and select an identifier for the peak as a time reference. This allows you to adjust for a shift in retention times.

  In Time-based heart cutting, the reference peak parameters are disabled until a reference peak has been identified in the Peak-based prameters.

• Reference Factor

  (Time-based Heart Cut)

  Enter a factor by which all Expected retention times are multiplied. This allows you to adjust for differing relative retention times.

  This parameter is disabled until a reference peak has been identified by Peak-based detection.

• Expected Time

  (Peak-based)

  Enter the expected elution time of the reference peak in this field.

• Reference Only

  (Peak-based)

  Mark this check box to indicate that this peak is a reference peak that should not be analyzed.

Parameter

(Time-based Comprehensive)

• Comprehensive Range

  Click in the cell to specify a stop time for comprehensive collection. Mark the Stop at check box and specify a time in minutes.

You can set up the Sampling Table either manually or interactively using the Preview. Any changes you make to the Sampling Table are synchronized with the Preview. For manual editing, the following buttons are also available:

Add	Inserts a line above the currently selected line in the Sampling Table.
Remove	Deletes the selected line in the Sampling Table.
Clear all	Clears the Sampling Table.
Cut	Deletes the currently selected line in the Sampling Table and places it on the clipboard.
Copy	Copies the currently selected line in the Sampling Table to the clipboard.
Paste	Pastes the line on the clipboard into the Sampling Table according to its time.