Reset Spray Chamber parameters (LC/MS)

If possible, get the values from the Autotune report that was shipped with the instrument listing its individual optimum values. If that is not available, use the following defaults.

Electrospray Spray Chamber Parameters

Parameter	Positive	Negative
Drying Gas Temperature (°C)	300 °C	300 °C
Drying Gas Flow (L/min)	7.0 L/min	6.0 L/min
Nebulizer Pressure (psi)	20 psi	20 psi
VCap (V)	4000 V	4000 V

APCI Spray Chamber Parameters

Parameter	Positive	Negative
Vaporizer (°C)	325 °C	325 °C
Corona (µA)	4 µA	4 µA
Drying Gas Temperature (°C)	300 °C	300 °C
Drying Gas Flow (L/min)	6.0 L/min	3.0 L/min
Nebulizer Pressure (psi)	15 psi	15 psi
VCap (V)	4000 V	2500 V

APPI Spray Chamber Parameters

Parameter	Positive	Negative
Vaporizer (°C)	300 °C	300 °C
Lamp	on	on
Gas Temperature (°C)	350 °C	350 °C
Gas Flow (L/min)	5.0 L/min	5.0 L/min
Nebulizer Pressure (psi)	15 psi	15 psi
VCap (V)	3000 V	3000 V

MMI-ES+APCI Spray Chamber Parameters

Parameter	Positive	Negative
Vaporizer (°C)	200 °C	200 °C
Gas Temperature (°C)	300 °C	300 °C
Gas Flow (L/min)	5 L/min	5 L/min
Nebulizer Pressure (psi)	40 psi	40 psi
VCap (V)	2000 V	2000 V
Corona (µA)	1 µA	1 µA
Charging Voltage (V)	2000 V	2000 V

MMI-ES  Spray Chamber Parameters

Parameter	Positive	Negative
Vaporizer (°C)	150 °C	150 °C
Gas Temperature (°C)	250 °C	250 °C
Gas Flow (L/min)	5 L/min	5 L/min
Nebulizer Pressure (psi)	60 psi	60 psi
VCap (V)	2000 V	2000 V
Corona (µA)	N/A	N/A
Charging Voltage (V)	2000 V	2000 V

MMI-APCI Spray Chamber Parameters

Parameter	Positive	Negative
Vaporizer (°C)	250 °C	250 °C
Gas Temperature (°C)	350 °C	350 °C
Gas Flow (L/min)	5 L/min	5 L/min
Nebulizer (psi)	20 psi	20 psi
VCap (V)	2000 V	2000 V
Corona (µA)	5 µA	5 µA
Charging Voltage (V)	2000 V	2000 V

AJS ESI Spray Chamber Parameters

Parameter	Positive	Negative
Gas Temperature (°C)	300 °C	300 °C
Gas Flow (l/min)	5 L/min	5 L/min
Nebulizer (psi)	45 psi	45 psi
Sheath Gas Temp (°C)	250 °C	250 °C
Sheath Gas Flow (L/min)	11 L/min	11 L/min
Capillary Voltage (V)	3500 V	3500 V
Nozzle Voltage (V)	500 V	500 V

1. Click Method.

2. In the Acquisition Method window in the navigation pane, click Instrument Setup > SQ.
3. If needed, click Take. In this image, Take is already selected.

4. In the navigation pane select Tune > Manual Tune.
5. In the navigation pane select Tune > Manual Tune > Source.

The source parameters are not saved with the tune file. They can be changed and downloaded by you to do some explicit manual tune experimentation, but these parameters will not be saved. The source parameters set in the method are saved and are what is used in acquiring a sample.

6. (optional) Open a different tune file. Click to open the Load Tune File dialog box. Select a tune file and click Load.
7. Click to lock the instrument for tuning. You need to unlock the instrument after you finish tuning.
8. If available, enter the Vaporizer. Refer to the tables above if you cannot find the paper shipped with the software.
9. Enter Gas Temp (°C).
10. Enter Gas Flow (L/min).
11. Enter Nebulizer (psi).
12. Enter Capillary Voltage (V).
13. If available, enter the Sheath Gas Temp (°C).
14. If available, enter the Sheath Gas Flow (l/min).
15. If available, enter the Corona (µA).
16. If available, enter the Charging Voltage (V).
17. Save the tune file.
18. Click to download these parameters to the instrument.
19. Click to unlock the instrument. You need to unlock the instrument after you finish tuning.

See Also

Tune the MS automatically (LC/MS)

Tune > Autotune/Checktune Section