Ramping Section (LC/MS)
Three of the sections in Tune include sections for ramping parameters: Tune > Manual Tune > Optics Section, Tune > Manual Tune > MS Section, and Tune > Manual Tune > Detector Section.
On each of these tabs, three different sections on ramping are available: Dynamic Ramp Table, Ramp, and Ramp Plots.
Dynamic Ramp Table
This section can be hidden. This section appears between the list of parameters and the Status section. You click on the arrow above the words Dynamic Ramp to show this table.
More than one parameter can be dynamically ramped in the same tune file, but the dynamic ramp for each parameter must be set up separately. To set up dynamic ramping of multiple parameters, select the first parameter and fill in all of the values in this section. Then, you can select a different parameter and do the same thing.
Dynamic ramping is the process of changing a parameter setting as the mass being analyzed changes. The software automatically does a linear interpolation between two adjacent data points. The parameter changes as the Mass (m/z) changes.
Parameter
Name of tune parameter for this dynamic ramp table.
Enabled
Whether or not to use the dynamic ramp table.
button
Adds a line to the table.
button
Deletes the selected line from the table.
Table
A set of Mass (m/z) and Value to use for each point in the Dynamic Ramp. You can only have up to six points in the table.
Ramp
Three of the sections in Tune include a section for ramping parameters:
- Tune > Manual Tune > Optics Section
- Tune > Manual Tune > MS Section
- Tune > Manual Tune > Detector Section
This section appears at the bottom of each of these sections.
Ramp Parameter
You can ramp only some of the tune parameters. If a parameter can be ramped, it is shown in blue in the user interface.
On a 6125C, 6130C, and 6135C, the following parameters can be ramped: Fragmentor, Skimmer, Octopole DC, Octopole RF, Lens1 DC, Lens2 DC, Lens2 DC RF Off, Lens2 RF, Lens2 RF Phase, MS DC, and Iris.
On a 6120C, the following parameters can be ramped: Fragmentor, Skimmer 2, Octopole DC, Octopole RF, Lens1 DC, Lens2 DC, MS DC, and Iris.
You can ramp any of these parameters from any section in Tune that allows you to ramp parameters.
From
The lower limit to use when ramping.
to
The upper limit to use when ramping.
by
The step size to use when ramping.
Settling time (ms)
The number of milliseconds to wait after changing the parameter by the step size.
Ramp button
Starts ramping the selected parameter.
Ramp Plots
Plot
When you click the Ramp button, the selected parameter is ramped over the selected range for each mass selected in the Profile masses section in the Tune > Manual Tune > MS Section.
Apply button
Sets the selected parameter to the point that you select in the plot. This button does not change the Dynamic Ramp table.
Clear button
Removes the current plot from the Plot window.
See Also
Tune > Manual Tune > Optics Section