This option is only available if your instrument is an Agilent 61xxC (for example, 6125C or 6135C).
The Autotune program adjusts the MS for good performance over the entire mass range. For many applications, there is no need to use anything else. You might consider manually tuning the MS in the following situations:
Manual Tune allows you to tune the SQ by adjusting one parameter at a time until the desired performance is obtained.
Separate tuning is not required for ESI, APCI/APPI, and MM-ESI+APCI. Tuning involves adjusting the parameters that control the transmission of ions. These parts neither know nor care how those ions are generated. A tune file created in electrospray mode often provides good results for APCI/APPI samples. The reverse is also true.
Separate tuning is required for positive and negative ionization. The MS must, at some time, be tuned twice, once with positive ions and once with negative ions. The two tunes can be stored under the same tune file name; the software keeps separate the positive and negative ion settings. Positive ion and negative ion settings can be updated separately.
What do you want to do?
Prepare for manual tuning (LC/MS)
User Interface
Tune > Manual Tune > Source Section (LC/MS)
Tune > Manual Tune > Optics Section (LC/MS)
Tune > Manual Tune > MS Section (LC/MS)