View profile scans (LC/MS)

When you are acquiring data, profile scans are visible in the Spectrum window. You do this procedure if you want to display the data in continuous (profile) mode rather than centroided.

1. Click Method.

2. In the Acquisition Method window in the navigation pane, click Instrument Setup > SQ.
3. If needed, click Spectrum.

4. In the navigation pane, select Tune > Acquisition.
5. If needed, click Take. In this image, Take is already selected.

6. (optional) Open a different tune file. Click to open the Load Tune File dialog box. Select a tune file and click Load.
7. Click to lock the instrument for tuning. You need to unlock the instrument after you finish tuning.
8.  Click the Polarity. You can click Positive or Negative.
9. Select Profile as the Acquisition type.
10. Enter the Samples and Averages for Profile.
11. Mark or clear the Centroid check box.
12. Click to start acquiring data.

In the Spectrum window, a profile scan is shown. You can continue to do other actions in Tune such as ramping parameters and changing values while you are acquiring data.

13. To stop acquiring data, click .
14. Click to unlock the instrument. You need to unlock the instrument after you finish tuning.

See Also

View Scan spectra (LC/MS)

Tune the MS manually (LC/MS)

Tune > Acquisition Section