Before you run a single sample, you must develop an acquisition method.
Enter the information in the Single Sample Analysis window before running the method; once the run has started, this information cannot be changed. The information in this window is not part of the method, and it is not saved when the method is saved. When you are running a sequence, you enter the information in the Sequence window.
Develop a method. See Method Editor for LC/MS Single Quadrupole.
Click Single Sample.
In the Single Sample Analysis window, enter the Run Information:
Enter the Sample name. The Sample name appears on reports that you may generate. Use the tokens to simplify this entry.
Select the Acq. method. This method is used to acquire the data. Click to open the Select an acquisition method dialog. An acquisition method has the extension .amx.
Select the Proc. method. This method is used to process the data. Click to open the Select a processing method dialog. A processing method has the extension .pmx.
Select the Result path. This path indicates where the data file will be saved. Click to open the Select Directory dialog.
Enter the Result name. Use the tokens to simplify this entry. A token is a parameter in the system. You can include any of the following in any order: Acq. method, Increment number, Instrument name, Local date & time, Proc. method, Sample name, Short local date & time, Username, and Vial. When you start the run, the file name is determined based on the parameters that you selected.
Enter the Autosampler information:
Select the Injection source.
Enter the Injection volume.
Enter the Vial.
Review the Processing Section. Click if the Processing Section is hidden.
(optional) Enter the Sample Description. Click if the Sample Description box is hidden.
If needed, click Take. In this image, Take is already selected.
Click Run.