Manually extract a signal (EIC or SIM ) from an injection

Privileges

• OpenLab CDS: Data Analysis > Do manual chromatogram extraction

• OpenLab CDS: Method Development > Edit chromatogram extraction parameters .
  Privileges are configured in the Control Panel.

Prerequisites

• If the project enforces method approval, the method status must be Generic. For details, see Method approval.

1. For EIC extraction (SCAN data):The m/z expansion range is set in the processing method. To change it, open the Processing Method window, and edit the parameters in the Extraction > Chromatogram node.

   The default m/z expansion is Asymmetric from -0.3 to +0.7.

2. In the Injection Tree, right-click the relevant injection, then select Extract Chromatograms... from the context menu.

   Alternatively, right-click any MS signal in the Chromatograms window, then select Extract Chromatograms... from the context menu.

   Enter the extraction parameters as required. The available values depend on the acquired data.

   

   Figure: Scan extraction parameters

   

   Figure: SIM extraction parameters

3. For EIC extraction: You can also enter m/z ranges.

   For example: 273,311 or 273,310.00-311.7 .

   NOTE

   Expansion parameters from the method affect only single m/z values.

4. To automatically reprocess the injection after extracting the chromatograms, select the corresponding check box.

   Peaks in the extracted ion chromatograms will be integrated during reprocessing.

5. Click OK.

   The EIC or SIM is extracted for the selected injection. It will be added to the list of signals for each injection, and can be shown or hidden the same way as other signals.

   

   

6. Save the data.

   Extracted chromatograms are saved together with the data.