UV Spectral Analysis

There are different windows and functions specific to UV spectral analysis. To view those windows and access the functions, the focused injection must contain spectral data (for example, acquired with a 3D UV system).

UV spectral analysis provides additional quality criteria for routine analytics:

• Confirm the compound identity

  The application compares a UV spectrum with a specific UV reference spectrum. A high match factor indicates that the compounds are probably identical.

  For details on the calculation, see UV confirmation.

• Check for UV impurities

  The application compares all UV spectra of a peak with the apex spectrum. It calculates an overall match factor, the UV Purity value. A low UV Purity value indicates that there are co-eluted peaks with a significantly different UV spectrum.

  For details on the calculation, see UV impurity check.

UV Spectral analysis processes spectral data acquired from a UV-visible diode-array detector or fluorescence detector. It adds a third dimension to your analytical data when using it with the chromatographic data.

Figure Spectral Information

If you installed the 2D-LC Add-on, UV spectral analysis offers additional functions. See 2D-LC help for details.