Isoabsorbance plot

What do you want to do?

• Pin and compare different graphs from the isoabsorbance plot

• Save extraction parameters to the processing method

• Zoom and scale the isoabsorbance plot

• Move zoomed section of a graph

• Change window proportions

• Use a different bandwidth

• Export isoabsorbance plot, chromatogram, or spectrum

• Adjust colors in the isoabsorbance plot

The Isoabsorbance plot window

The isoabsorbance plot gives an overview of retention times and UV spectra for injections with 3D UV signals. If different isoabsorbance plots are provided, you can select the detector in the toolbar of the isoabsorbance plot.

• The x-axis shows the retention time

  If a signal uses a delay (see Align MS and UV Chromatograms), the updated retention time is shown.

• The y-axis shows the wavelength

• The color reflects the response, that is, the UV absorbance.

  Note that blank subtraction is not taken into account.

At the bottom of the isoabsorbance plot, the extracted chromatogram is shown for a specific wavelength.

To the right of the isoabsorbance plot , the spectrum is shown for a specific retention time. The wavelengths of peak apexes (λ_max) are annotated.

Drag the dashed lines to view the corresponding chromatograms and UV spectra. All graphs are synchronized.