Edit Auto Acquire section (LC/MS)

In the Auto Acquire user interface, all of the parameters for the SQ are shown in the same pane.

The columns available in the Scan Table change depending on whether the row has a Scan type of Scan or SIM.

You can add rows to the Acquisition Parameters table, delete rows, copy values, copy rows, move a scan segment, and copy a scan segment.

If needed, show the Auto Acquire section.
1. Click Method.
2. In the Acquisition Method window in the navigation pane, click Instrument Setup > SQ.
3. In the navigation pane select Auto Acquire. If this section is grayed out, then the current method cannot be used with the Auto Acquire user interface. See Edit Advanced Acquire section (LC/MS).
Click the Stop time. You can click either As pump/No limit or Limit (min).
Edit the Acquisition Parameters section:
1. Enter the following information for a Scan row. You can have up to 4 Scan rows in the table. These 4 scans are acquired throughout the entire run. You do not specify a Time.
  - Select Scan for the Scan type.
  - Select the Polarity. An error is shown if the selected polarity does not match the autotuned polarities of the instrument.
  - (optional) Enter the Compound/Segment name. Compound name is used if you wish to automatically build a quantitation method in Data Analysis.
  - Enter the Mass range start (m/z).
  - Enter the Mass range end (m/z).
2. Enter the following information for a SIM row. You can have up to 500 SIM rows for a SIM only method, or up to 500 total rows with up to 4 Scan rows for a SIM/Scan method.
  1. Select SIM for the Scan type.
  2. Select the Polarity. An error is shown if the selected polarity does not match the autotuned polarities of the instrument.
  3. (optional) Enter the Compound/Segment name.
  4. Enter the Mass (m/z).
3. If you want to add a row at the end of the table, click . A new row is added at the end of the table. It has the same values as the row that was selected. You can add up to 4 Scan rows.
4. If you want to insert a row in the middle of the table, first highlight a row, and then click . A new Compound/Segment row is added after the highlighted row. It has the same values as the row that was selected. You can add several rows.
5. For a new Scan row, enter the Polarity, Compound/Segment name, Mass range start (m/z), and Mass range end (m/z).
6. For a new SIM row, enter the Polarity, Compound/Segment name, and Mass (m/z).
7. Select Centroid or Profile for the Data storage. This parameter is shown below the table.
8. Enter the Targeted points per second (Hz). You can then select the Actual targeted points per second (Hz) value from the list.

The Estimated cycle time (ms/cycle) is set automatically based on the number of compounds and the internal Scan/Dwell times.

The SIM (%) value is shown below the Acquisition Parameters table. It is a read-only value that shows the percentage of each cycle which is used by SIM.

Edit the Chromatograms section.
1. Select the Chrom type. You can select TIC, Channel TIC, EIC, or BPC.
  - TIC - total ion chromatogram. The TIC is the overlay of all of the TICs of each scan segment. By default, there is one TIC per method for chromatograms.
  - Channel TIC - a total ion chromatogram for a single scan segment. The channels correspond with the scan segments.
  - EIC - extracted ion chromatogram.
  - BPC - base peak chromatogram.
2. If you select EIC (extracted ion chromatogram), enter the Extracted mass (m/z). You can enter a range by using a dash. For example, you can type 300 - 350.
3. If you select BPC (base peak chromatogram), enter the Excluded masses (m/z) or leave it blank so that no mass is excluded in the BPC. You can enter a range by using a dash. For example, you can type 100 - 200. Multiple individual masses or mass ranges may be entered. Each must be separated by a ";" (semi-colon). For example, you can type 300; 400 - 500.
4. To add another row to the table, click .
5. To delete a row from the table, select the row and then click .

Edit the Timetable section.
1. Enter the Start Time (min).
2. Select the Type. You can select Diverter.
3. Select the Value. You can select either To MS or To waste.
4. Select or clear Post-run diverter position which is below the table. If Post-run diverter position is selected, you select either To MS or To waste.
5. To add another row to the table, click .
6. To delete a row from the table, select the row and then click .

Other actions on the Acquisition Parameters table.

If you want to delete a row, first highlight the row, and then click .
If you want to copy one value to all of the other cells in a column, first select the cell that you want to copy. Then, click . The selected value is copied to all of the rows in the table.
If you want to copy one value to another cell, do the following:
1. Select the cell that you want to copy.
2. Right-click and select Copy.
3. Click the cell where you want to copy the value.
4. Right-click and select Paste.

If you want to copy a scan segment to another location in the table, do the following:
1. Select the row that you want to copy.
2. Click .
3. Select the row adjacent to where you want to move the row.
4. Click to insert a row in the table.
5. Select the row that you want to replace.
6. Click .

Edit Auto Acquire section (LC/MS)

Other actions on the Acquisition Parameters table.

See Also