The Lens2 RF is not equipped on the 6120C instrument.
Before you tune the Lens2 RF parameter, you tune the instrument with the Lens2RF Enabled check box cleared. It is important to know that the Lens2RF parameters do affect the resolution of the mass filter. In general, the resolution will improve for all masses if properly tuned, with the effect most noticeable at the higher masses and less so at the lower masses.

to
lock the instrument for tuning. You need to unlock the instrument
after you finish tuning.See Also
Tune the MS automatically (LC/MS)