Run Checktune (LC/MS)
If Checktune is Out of Tolerance, please run an Autotune. See Tune the MS automatically (LC/MS).
Fast scan Checktune is not supported.
- Click Method.

- In the Acquisition Method window in the navigation pane under Instrument Setup, click SQ.
- In the navigation pane, select Tune > Autotune.
- If needed, click Take. In this image, Take is already selected.

- (optional) Open a different tune file. Click
to open the Load Tune File dialog box. Select a tune file and click Load.
- Click
to lock the instrument for tuning. You need to unlock the instrument after you finish tuning.
- Click the Polarity. You can click Positive, Negative, or Both.
- (optional) Click Spectrum. Some graphs are displayed in the Spectrum window when you run a Checktune.

- Click
. Checktune will run and a report is generated.
- Click
to unlock the instrument. You need to unlock the instrument after you finish tuning. If you do not unlock the instrument, it is unlocked automatically after 3 - 4 minutes.
See Also
Tune the MS automatically (LC/MS)
Tune the MS using Fast scan (LC/MS)
Tune > Autotune/Checktune Section