Set acquisition parameters and adjust mass axis (LC/MS)

The purpose of this step is to perform a preliminary calibration of the mass axis to ensure that the mass peaks are within their assigned windows.

1. In the navigation pane, select Tune > Manual Tune.
2. In the navigation pane select Tune > Manual Tune > Acquisition.
3. If needed, click Take. In this image, Take is already selected.

4. If necessary, click to lock the instrument for tuning. You need to unlock the instrument after you finish tuning.
5. Enter 8 for Samples in the Profile column.
6. Enter 9 for Averages in the Profile column. Do not change any other values.
7. In the navigation pane under Tune, select MS.
8. In the Profile masses table, set the Window (u) to 25 for each mass.
9. Click the Adjust Mass Peaks button. The Width gain, the Width offset, the Axis gain, and the Axis offset are adjusted.
10. In the Profile masses table, set the Window (u) to 5 for each mass.

 You can look at the Spectrum window to look at the peaks as they are adjusted.

See Also

Manually tune the MS (LC/MS)

Tune the MS automatically (LC/MS)

Tune the MS using Fast Scan (LC/MS)

Tune > Manual Tune Section (LC/MS)