Configure processing method for 2D UV impurity check

Preparations

• To be able to carry out the procedure as described, you need the privilege Processing Method > Edit spectra parameters . Privileges are configured in the Control Panel.

• If the project enforces method approval, the method status must be Generic. For details, see Method Approval.

1. In the Processing Method window, select the Compounds > Spectra ²D node.

2. Check the settings in the UV Impurity Check tab. By default, the same settings as in the 1st dimension are used. To use different settings, clear the Use same parameters (¹D) Spectra check box.

   • Calculate UV Purity

     Select to calculate values for all integrated peaks or for identified 2D peaks only. Select None if you do not want to calculate values for any 2D peaks.

   • Lower wavelength [nm], Upper wavelength [nm]

     Wavelength range to use for the impurity check in spectra of ²D peaks . By default, the whole range is used.

   • Sensitivity unidentified peak [%]

     By changing the sensitivity value (default: 50 %), you influence the Threshold calculation and thus the criteria for a green or red coloring of the UV Purity value in the injection results.

   • Threshold exceptions

     Number of data points in a row that are allowed below the threshold line. See Adapt the threshold exceptions.

3. In the Compound Table tab, adjust the sensitivity for identified compounds in the Impurity sensitivity [%] column.

4. Click Reprocess All.

UV purity results are shown in the following windows:

• For individual ²D peaks: in the Peak Table ²D, Chromatograms ²D, or Peak Details window

• For 2D peaks: in the Injection Results or Contour Plot 2D window

• For ¹D peaks: in the Chromatograms or Peak Details window