Quantify compounds from 2nd dimension

Preparations

• To be able to carry out the procedure as described, you need the privilege Project Management > Edit content of project . Privileges are configured in the Control Panel.

1. Load the raw 2D data.

2. Make sure there is a 2D-LC method linked to the data (method configuration: 2D-LC).

3. Make sure that the peaks in the 2nd dimension are integrated correctly.

   For details, see Optimize integration in the 2nd dimension by adjusting integration parameters.

4. Make sure that all peaks found in the ²D chromatogram are assigned correctly.

   For details, see Optimize 2D peak assignment by adjusting the method.

5. Add the 2D peaks as 2D compounds to the processing method.

   For details, see From the Contour Plot 2D.

6. Set up a calibration for the 2D compounds.

   Setting up a calibration works identically for 1D and 2D compounds. For details, see Set up a calibration.

   The only restrictions for 2D compounds are the following:

   • If you quantify a 2D compound with a calibration curve of another compound, the other compound must be a 2D compound as well. To quantify a 1D compound, the other compound must be 1D as well.

   • If you use internal standards for 2D compounds, you can only assign 2D internal standards. For 1D compounds, you can only assign 1D internal standards.

7. Reprocess your data.

   The Calibration Curve window shows the calibration curve for a selected compound.

   The Injection Results window shows the amount and concentration. Injection results include results from both 1st dimension and 2nd dimension. The corresponding signal name is visible in the Signal description column.

   Note that for 2D peaks, area and height are calculated differently than for 1D peaks. See Properties of a 2D peak.

   For more information on results, see Results in the core Data Analysis help.

See Also

Calibration Curves