Sequence table columns

The following columns are available in the sequence table.

Table: Sequence table columns

Column

Description

Allowed value and range

Action

An indication of the action performed at that line of the sequence. The value is read only. Actions include Inject (perform an injection) and Create report (no injection, but the specified data will be processed, and a sequence summary report will be generated).

Acq. method

The name of the method used to acquire the raw data (acquisition method). If using a method in a subfolder, the subfolder path relative to the root path must be used. For example, <sub-folder>\<method file name>.

Any character, no limit

Barcode

The barcode label as it has been read from the sample vial. The value is read only and only intended for automation purposes. See Check barcodes during a run.

Any character, no limit

Bracket

Displays how calibration standards are bracketed according to the selected Bracketing mode. This column is populated only when a Sequence Creation Template is applied that contains a bracketing mode. This column cannot be imported into the sequence table from a CSV or TSV file.

Any character, no limit

Compound amounts

An indication if the amount of a calibration sample has been modified. The compound amount is defined in the calibration parameters in the method, but may be adjusted for individual injections. When clicking in this column for a calibration standard, the window shows the actual amounts in the sample and amounts defined in the method.

Numbers only, 7decimal.9decimal limit, for example: 1234567.123456789

Custom parameters

If you have configured custom fields for your project in Control Panel, you can view and edit the values in this column. Click in the column to view Sample and Compound custom parameters and values. Whether or not a customer parameter is mandatory is specified in Control Panel. Values of mandatory parameters cannot be deleted. This column cannot be imported into the sequence table from a CSV or TSV file.

Text: characters only

Number: numbers only

Date/time: entry format and validation is set by local Windows date/time format

Data file

The name of the individual injection's .dx file that gets saved to storage. You can manually change the data file name; however, you cannot have duplicate file names.

Default value uses the token <D>-<##>. For example 2017-03-13 10-17-58-07-00-01.

255 characters, special characters will be resolved to - at runtime

Description

A description of the sample.

4096 character limit

Dil. factor1

Dil. factor2

Dil. factor3

Dil. factor4

Dil. factor5

The dilution factor per sample. Used to calculate the concentration for all compounds.

Numbers only, 5decimal.5decimal limit, for example: 12345.12345

Expected barcode

The barcode entered manually by running a manual scan of the vials or by typing the barcode value directly into the column. See Check barcodes during a run.

Any character, no limit

Frac. Recovery Location

The location in the recovery device where the eluate is stored after a fraction is collected. This column is only available when your instrument configuration contains a fraction recovery device.

Any valid vial location. Allowed values determined by fraction recovery device specifications.

Frac. Start Location

The vial number in the fraction collection tray where the first fraction is collected. This column is only available when your instrument configuration contains a fraction collection device.

Any valid vial location. Allowed values determined by fraction collection device specifications.

Inj/Vial

The number of times a line in the sequence will be run. For example, a value of 3 for line 1 in the sequence table will re-run line 1 three times and generate 3 different data files.

Numbers only, 999 character limit

Injection source

The location where the sample will be injected. Options are instrument dependent. This column cannot be imported into the sequence table from a CSV or TSV file.

Select from available options

ISTD amt1

ISTD amt2

ISTD amt3

ISTD amt4

ISTD amt5

The amounts of each of the internal standards being used in the analysis. Values entered in the sequence table override the ISTD amount in the method. If no value is entered, the amount in the method is used.

Numbers only, 5decimal.5decimal limit, for example: 12345.12345

Label selection

The label for an injection run. Used to create sequence summary reports.

Alphanumeric characters only

Level

For calibration standards, the level of the standard.

Numbers only, 99 character limit

LIMS ID1

LIMS ID2

LIMS ID3

The keys required for connection to and from a LIM system.

Any character, no limit

Multiplier1

Multiplier2

Multiplier3

Multiplier4

Multiplier5

The multiplication factor per sample. Used to calculate the concentration for all compounds.

Numbers only, 5decimal.5decimal limit, for example: 12345.12345

Proc. method

The name of the method used to analyze the data (processing method). If using a method in a subfolder, the subfolder path relative to the root path must be used. For example, <sub-folder>\<method file name>.

Any character, no limit

Run type

  • Clear all calibration: All previous calibration points will be removed when processing this standard.

  • Clear Calibration at level: All previous calibration points of the level of the calibration standard will be removed when processing this standard.

  • Empty: A new point will be added to the calibration curve. If a calibration point already exists for the current injection, the application calculates the average value of all existing calibration points for the given target.

Select from available options

Sample amount

The weight of the sample before dilution.

Numbers only, 5decimal.5decimal limit, for example: 12345.12345

Sample name

The name of the sample.

255 character limit

Sample prep method

The name of the method used to prepare the sample. If using a method in a subfolder, the subfolder path relative to the root path must be used. For example, <sub-folder>\<method file name>.

Any character, no limit

Sample type

The type of sample.

Select from available options

Target1

Target2

Target3

Target4

Target5

For mass spectrometry, the masses or formulas that you want to use for the MS sample purity calculation. You can mix molecular weight and formulas, for example, C12 H14 N4 O4 S, 284.

Validation is case sensitive

Vial

The vial number or plate location.

Configuration-dependent, validated by instrument

Volume

The volume of the sample you want to inject at run time. Usually defined in your acquisition method, you can manually override your method injector volume settings in this column. Units of measurement are defined by the instrument configuration.

Configuration-dependent, validated by instrument

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