Working with 2D-LC methods

What do you want to do?

• Learn more about processing methods for 1D data in the core Data Analysis software

• Learn more about method configurations

NOTE

Processing methods typically contain many time-based parameters, such as expected retention times or retention time windows. Such methods are designed for data that had been acquired with a certain flow, column dimension and other acquisition parameters.

If you want to apply such methods to other data with different acquisition parameters, you must adapt the method settings.

2D-LC methods

The selected method configuration determines which sections of the method are visible and can be edited in the Processing Method window.

2D-LC methods contain the following sections:

• General:

  • Properties section: Same function as for 1D data.

  • Signals section:

    In the Alignment tab, you adjust the delay of your ¹D and ²D detectors (see Align signals over two dimensions).

    In the Blank Subtraction tab, you specify how the ¹D signals of blank runs will be subtracted from the ¹D signals of sample runs.

    In the Blank/Cut Subtraction ²D tab, you specify how the ²D signals of blank runs will be subtracted from the ²D signals of sample runs.

    The settings in the Smoothing and Reference Chromatogram tabs only apply to ¹D signals.

• Extraction:

  Under Extraction, you specify the parameters for:

  • MS and UV chromatogram extraction from ¹D data

    MS Chromatogram extraction from ²D data (see Extract Chromatogram for MS data recorded in ²D)

  • MS and UV spectra extraction from ¹D data

    MS and UV spectra extraction from ²D data (see Extract an MS spectrum from a ²D signal, and Extract a UV spectrum from a ²D signal)

• Integration Events: Same function as for 1D data

• 2D Peaks:

  See Optimize 2D peak assignment by adjusting the method.

• Compounds:

  • Identification section, Compound Table tab: Same function as for 1D data, plus renamed and additional values.

    Qualifier Setup tab: These settings apply only to ¹D signals.

    General tab: Additional option to use ²D time reference compounds.

  • Calibration section:

    Same function as for 1D data.

  • Spectra section:

    These settings apply only to ¹D signals.

  • Spectra ²D:

    Check ²D and 2D peaks for UV impurities

• System Suitability

  Same calculations as for 1D data. However, there are a few differences in the workflow and output. See System Suitability and Noise calculation.

• Reports

  Same function as for 1D data. Choose a 2D-LC report template.

• Custom Calculation

  Same function as for 1D data, plus 2D-LC fields and functions.