Run a sequence

1. Click Sequence, and then click Table.

   

2. Complete the Sequence table using one or more of the following techniques:

   • Reuse and modify a saved sequence

   • Create a sequence manually

   • Create a Dual Simultaneous sequence

   • Apply a sequence template

   • Import a CSV or TXT file

3. Optional: Add barcodes to the sequence table.

4. Optional: Select a printer to override the printer specified in the processing method.

5. Select the rows to submit as part of the run. By default, all rows are selected.

6. To save your results to a sub-folder inside your Results folder, change the Result path by clicking Browse and creating a new folder. You cannot save your results to an existing .rslt folder.

   

7. The Result name defaults to the last filename used for this instrument and project. Enter a filename by typing in the field or using the token button. If left blank, the instrument name and local date & time is used for the filename.

8. Select Save result as:

   • One result set to save the data acquired for all sample injections in the sequence within a single result set. For example:

     ResultSetName: A1, B1, A2, B2, A3, B3, A4, B4, A5, B5

   • Separate single injections to save each sample injection in the sequence as an individual result set. When using this option, result set names will be based on the value of the data file column for each sequence row. For example:

     DataFileName: A1

     DataFileName: B1

     DataFileName: A2

     DataFileName: B2

9. Optional: Click Validate to validate the sequence against the specified acquisition method. The columns validated are Acq. method, Volume, and any override parameters. If invalid entries are found, correct those entries.

   For some LC instruments, validation also predicts whether the existing solvent quantities are sufficient to complete the sequence (including any queued runs that use the same resources).

10. Click Run. If this is a new "Untitled" sequence, the sequence file is saved as the instrument name + the local date and time. Submitted sequences are placed in the Run Queue, where you can stop or pause a run, or edit a sequence. A result folder (.rslt) is created when the run is added to the Run Queue.

    

See Also

Display the status of a sequence

Modify a submitted sequence

Stop a submitted sequence or single-sample run

Validate a sequence

Create a Dual Simultaneous sequence